Journal: BioMed Research International

Article Title: Proteomic Analysis of Fetal Ovaries Reveals That Primordial Follicle Formation and Transition Are Differentially Regulated

doi: 10.1155/2017/6972030

Figure Lengend Snippet: Representative 2D-DIGE gel image of differentially expressed proteins of fetal ovaries at day 55 and day 90 of gestation. The proteins extracted from the fetal ovaries at day 55 and day 90 of gestation samples were labelled with cy3 and cy5, respectively. An internal standard protein sample (a mixture of fetal ovaries at day 55 and day 90 of gestation samples) was labelled with the Cy2 dye. The number in the figure corresponds to the number shown in .

Article Snippet: The 2D DIGE gel images were analyzed by the Image Master 2D platinum 7.0 software (GE Healthcare Life Sciences, NJ, USA) and the protein abundance changes for spot picking detection were calculated using cy3/cy2 and cy5/cy2 differential in-gel analysis ratios.

Techniques:

Journal: Imaging Science in Dentistry

Article Title: The Vectra M3 3-dimensional digital stereophotogrammetry system: A reliable technique for detecting chin asymmetry

doi: 10.5624/isd.20210168

Figure Lengend Snippet: Soft tissue changes of the soft pogonion with deviations of the dental midline using indices of 1-4 mm. The values are based on VECTRA® M3 auto tracking.

Article Snippet: Unlike traditional 2-dimensional images, which are taken by 1 camera in a single view, the Vectra® M3 imaging system consists of 6 cameras that simultaneously capture the face from different angles to render a 3D image that offers new possibilities for planning and evaluating treatments.

Techniques:

Journal: BMC Plant Biology

Article Title: Integration of proteomic and genomic approaches to dissect seed germination vigor in Brassica napus seeds differing in oil content

doi: 10.1186/s12870-018-1624-7

Figure Lengend Snippet: The 2D-DIGE maps and the functional categorization of DEPs in germinating B. napus seeds with high and low oil content. a - e represent the 2D-DIGE maps of 12WH191 (H) and KenC8 (L) germinating seeds at 0, 12, 24, 36 and 48 HAI. f represents the functional categorization of DEPs. Arrows show the protein spots that were highly expressed in low oil-containing seeds; lines show the protein spots that were highly expressed in high oil-containing seeds

Article Snippet: For 2D-DIGE analysis, the labeled proteins were mixed with 2D-DIGE buffer (7 M urea, 2 M thiourea, 4% CHAPS, 0.4% DTT, 0.5% IPG buffer) and separated with isoelectric focusing after being loaded on an immobilized pH gradient strip (IPG, pH 4–7, 24 cm; Amersham Biosciences, Uppsala, Sweden) [ ].

Techniques: Functional Assay